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Image Search Results
Journal: Tissue Engineering. Part A
Article Title: The Human Tissue–Biomaterial Interface: A Role for PPARγ-Dependent Glucocorticoid Receptor Activation in Regulating the CD163 + M2 Macrophage Phenotype
doi: 10.1089/ten.tea.2013.0628
Figure Lengend Snippet: The PPARγ-dependent regulation of the macrophage costimulatory receptor CD80. Human peripheral blood monocyte–derived macrophages expressed a basal level of CD80 when in the presence of the DMSO vehicle control for 11 days. Inhibition of PPARγ by culture of monocyte-derived macrophages in the presence of 5 μM T0070907 for 11 days on a glass substrate showed a qualitative increase in CD80 expression. Activation of PPARγ by culture of the monocyte-derived macrophages in the presence of the PPARγ agonist troglitazone at 1 μM for 48 h, followed by culture in the DMSO vehicle control medium for 9 days, showed a loss in CD80 expression by monocyte-derived macrophages. Scale bar represents 100 μm. Color images available online at www.liebertpub.com/tea
Article Snippet: THP-1 cells were differentiated to adherent macrophage-like cells by culture in 100 nM PMA for 48 h. Primary antibodies The phenotype of mononuclear phagocytes was assessed using mouse monoclonal antibodies against CD11b (Clone 238446, MAB16991; R&D Systems), CD11b-APC (Clone 238446, FAB16991A; R&D Systems), CD68 (Clone PG-M1, M0876; Dako),
Techniques: Derivative Assay, Control, Inhibition, Expressing, Activation Assay
Journal: Tissue Engineering. Part A
Article Title: The Human Tissue–Biomaterial Interface: A Role for PPARγ-Dependent Glucocorticoid Receptor Activation in Regulating the CD163 + M2 Macrophage Phenotype
doi: 10.1089/ten.tea.2013.0628
Figure Lengend Snippet: Expression of CD80 and localization of PPARγ in monocyte-derived macrophages seeded onto either a glass substrate or the PABM. Arrows denote macrophages on PABM showing nuclear PPARγ localization. Scale bar represents 100 μm. Color images available online at www.liebertpub.com/tea
Article Snippet: THP-1 cells were differentiated to adherent macrophage-like cells by culture in 100 nM PMA for 48 h. Primary antibodies The phenotype of mononuclear phagocytes was assessed using mouse monoclonal antibodies against CD11b (Clone 238446, MAB16991; R&D Systems), CD11b-APC (Clone 238446, FAB16991A; R&D Systems), CD68 (Clone PG-M1, M0876; Dako),
Techniques: Expressing, Derivative Assay
Journal: Life sciences
Article Title: TPX2 influences the regulation of macrophage polarization via the NF-κB pathway in lung adenocarcinoma.
doi: 10.1016/j.lfs.2024.122437
Figure Lengend Snippet: Fig.2 Expression of TPX2 in A549 cells affects macrophage polarization. (A) Detection of macrophage surface markers CD80 and CD163 after cell supernatant induction in A549 group, Vehicle group and TPX2-shRNA group. (B) The analysis percentage of macrophage surface marker CD80, CD163; CD80/CD163 after induction by cell supernatant of the three groups. (C) CD80 and CD163 protein expression in macrophages after induction in the supernatant of the three groups cells. (D) The
Article Snippet: THP-1 cell differentiation into macrophages was induced by culturing for 24 h. The supernatant of each group was then applied for 48 h. The induced cells were digested and washed twice, followed by the addition of
Techniques: Expressing, shRNA, Marker